Users should refer to the original published version of the material for the full abstract. No warranty is given about the accuracy of the copy. However, users may print, download, or email articles for individual use. Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission.Enhanced galactosylation and sialylation was shown for some of the Mabs tested.Cotton-based hydrolysates showed high bioactivity and potential for use in serum-free media.(e.g., IVCD, Qp, consumption rates) and multi-parametric assessment of any type of time-series. Protein hydrolysates enhanced both IVCD & qMab the effect on qMab being consistently greater. Leveraging these factors along with other cell culture.The cotton-based hydrolysates can be viewed as valuable supplements for animal-derived component-free (ADCF) media and as a source for the investigation of chemically defined bioactive components. Overall, the animal hydrolysate, Primatone and two cotton-derived hydrolysates provided the most substantial benefit for enhanced productivity. This showed no change in the predominant seven glycans produced but a significant increase in the galactosylation and sialylation of some but not all the antibodies. The effect of one of the most active hydrolysates (HP7504) on antibody glycosylation was investigated. There were individual differences between hydrolysates in terms of enhancement of mAb productivity, the highest being a 166% increase of mAb titre (to 117 mg/L) in batch cultures of CHO-EG2 supplemented with UPcotton hydrolysate. There was a marginal but significant overall increase (x1.1) in the integral viable cell density (IVCD) in the presence of hydrolysate but a more substantial increase in the cell-specific mAb (qMab) productivity (x1.5). Differences between cultures appeared in the decline phase which was followed up to 7 days beyond the start of the cultures. In all cultures, a consistent growth rate was shown in batch culture up to 4 to 5 days. This generated a 7x4 matrix of replicate cultures which was analysed for viable cell density and mAb productivity. Abstract: Four independent mAb-producing CHO cell lines were grown in media supplemented with one of seven protein hydrolysates of animal and plant origin.
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